Hotspot

petG-trnW-CCA

LSC · 69087-69217 · 131 bp

Back to Grewia

Pi 0.00513
MarkerSeek score 57.30
Primer available yes
Label petG-trnW-CCA

Feature Metadata

label_name
petG-trnW-CCA
feature_type
igs
region
LSC
length_bp
131
variable_sites
1
pi
0.00513
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petG-trnW-CCA_p1 TGGCTGGCTTATTCGTAACC AGCGCTCTTAGTTCAGTTCG 50.0 50.0 58.0 58.0 266-268 bp 1.0 80.5
petG-trnW-CCA_p2 TGGCTGGCTTATTCGTAACC AGAACGTGGGTCTCCAAAAC 50.0 50.0 58.0 58.0 244-246 bp 1.0 80.4
petG-trnW-CCA_p3 ACAGACGTGGTGATCAGTTG AGCGCTCTTAGTTCAGTTCG 50.0 50.0 57.8 58.0 233-235 bp 1.0 79.7
petG-trnW-CCA_p4 ACAGACGTGGTGATCAGTTG AGAACGTGGGTCTCCAAAAC 50.0 50.0 57.8 58.0 211-213 bp 1.0 79.6
petG-trnW-CCA_p5 ACTTTGGCTGGCTTATTCGT AGCGCTCTTAGTTCAGTTCG 45.0 50.0 57.8 58.0 270-272 bp 1.0 79.2