Hotspot

petA-psbJ

LSC · 65954-67099 · 1146 bp

Back to Grewia

Pi 0.00582
MarkerSeek score 63.60
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1146
variable_sites
10
pi
0.00582
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CTACGTGTCCAAGGCCTTTT GGCACGGTAACTGGTATTCC 50.0 55.0 58.1 58.3 1338-1344 bp 1.0 77.7
petA-psbJ_p2 GGCCTTTTGTTCTTCTTGGC GGCACGGTAACTGGTATTCC 50.0 55.0 57.8 58.3 1326-1332 bp 1.0 77.4
petA-psbJ_p3 CTACGTGTCCAAGGCCTTTT ATTCTGGATTGGGTTCGTCC 50.0 50.0 58.1 57.6 1280-1286 bp 1.0 77.1
petA-psbJ_p4 GGCCTTTTGTTCTTCTTGGC ATTCTGGATTGGGTTCGTCC 50.0 50.0 57.8 57.6 1268-1274 bp 1.0 76.8
petA-psbJ_p5 TACGTGTCCAAGGCCTTTTG GGCACGGTAACTGGTATTCC 50.0 55.0 58.4 58.3 1337-1343 bp 1.0 75.4