Hotspot

trnG-UCC-trnR-UCU

LSC · 9763-9960 · 198 bp

Back to Grewia

Pi 0.00337
MarkerSeek score 36.20
Primer available yes
Label trnG-UCC-trnR-UCU

Feature Metadata

label_name
trnG-UCC-trnR-UCU
feature_type
igs
region
LSC
length_bp
198
variable_sites
1
pi
0.00337
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
36.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 279 bp 1.0 63.6
trnG-UCC-trnR-UCU_p2 CCTAGCCTTCCAAGCTAACG AGAAGACCTCTGTCCTATCCA 55.0 47.6 58.1 56.9 282 bp 1.0 63.4
trnG-UCC-trnR-UCU_p3 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 285 bp 1.0 63.0
trnG-UCC-trnR-UCU_p4 CCTAGCCTTCCAAGCTAACG AGGTTTAGAAGACCTCTGTCCT 55.0 45.5 58.1 57.9 288 bp 1.0 62.7
trnG-UCC-trnR-UCU_p5 CCCTAGCCTTCCAAGCTAAC AGAAGACCTCTGTCCTATCCA 55.0 47.6 57.7 56.9 283 bp 1.0 61.2