Hotspot

trnS-GCU-trnS-CGA

LSC · 8185-8959 · 775 bp

Back to Grewia

Pi 0.00946
MarkerSeek score 51.20
Primer available yes
Label trnS-GCU-trnS-CGA

Feature Metadata

label_name
trnS-GCU-trnS-CGA
feature_type
igs
region
LSC
length_bp
775
variable_sites
11
pi
0.00946
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnS-CGA_p1 CAATCCGACGCTTTAGTCCA ATGAATCAAACCGAGGGACC 50.0 50.0 58.0 57.6 890-891 bp 1.0 77.6
trnS-GCU-trnS-CGA_p2 AATCCGACGCTTTAGTCCAC ATGAATCAAACCGAGGGACC 50.0 50.0 58.0 57.6 889-890 bp 1.0 77.6
trnS-GCU-trnS-CGA_p3 CGCACAACGGATTAGCAATC ATGAATCAAACCGAGGGACC 50.0 50.0 57.9 57.6 905-906 bp 1.0 76.9
trnS-GCU-trnS-CGA_p4 ACGGAAAGAGAGGGATTCGA ATGAATCAAACCGAGGGACC 50.0 50.0 58.2 57.6 944-945 bp 1.0 76.4
trnS-GCU-trnS-CGA_p5 TCGCACAACGGATTAGCAAT ATGAATCAAACCGAGGGACC 45.0 50.0 58.3 57.6 906-907 bp 1.0 75.5