Hotspot

rps16-trnQ-UUG

LSC · 6356-6892 · 537 bp

Back to Grewia

Pi 0.00621
MarkerSeek score 65.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
537
variable_sites
5
pi
0.00621
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AGTGGTGGGTTTTGACATCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 632-649 bp 1.0 82.3
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 593-610 bp 1.0 81.5
rps16-trnQ-UUG_p3 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 604-621 bp 1.0 80.9
rps16-trnQ-UUG_p4 GTGGTGGGTTTTGACATCCA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.3 58.1 631-648 bp 1.0 80.0
rps16-trnQ-UUG_p5 TGGTGGGTTTTGACATCCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.3 58.1 630-647 bp 1.0 80.0