Hotspot

psbA

LSC · 309-1370 · 1062 bp

Back to Gravesia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TCAAATGATTATTGCTCCCCC	AGAATTCGTGTGCTTGGGAG	42.9	50.0	56.0	58.2	1191 bp	1.0	54.2
psbA_p2	TCAAATGATTATTGCTCCCCC	AGAGAATTCGTGTGCTTGGG	42.9	50.0	56.0	58.2	1193 bp	1.0	54.2
psbA_p3	TCAAATGATTATTGCTCCCCC	GAGAATTCGTGTGCTTGGGA	42.9	50.0	56.0	58.2	1192 bp	1.0	54.1
psbA_p4	TCAAATGATTATTGCTCCCCC	TGGGAGTCCCTGATGATGAA	42.9	50.0	56.0	57.7	1177 bp	1.0	53.5
psbA_p5	TCAAATGATTATTGCTCCCCC	TTGGGAGTCCCTGATGATGA	42.9	50.0	56.0	57.7	1178 bp	1.0	53.5