Hotspot

atpI

LSC · 14689-15432 · 744 bp

Back to Gravesia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: LSC
length_bp: 744
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	CCAAGTAGATTATCTTGAATTCCGC	GCAAGTCGCAAAAGAGATGG	40.0	50.0	58.1	57.7	882 bp	1.0	40.0
atpI_p2	TGAATTCCGCTATTATTTTGGTCT	GCAAGTCGCAAAAGAGATGG	33.3	50.0	56.7	57.7	867 bp	1.0	40.0
atpI_p3	ACTGCCTTATTAGTTATCCCT	GCAAGTCGCAAAAGAGATGG	38.1	50.0	53.4	57.7	928 bp	1.0	40.0
atpI_p4	TGAATTCCGCTATTATTTTGGTC	GCAAGTCGCAAAAGAGATGG	34.8	50.0	55.4	57.7	867 bp	1.0	40.0
atpI_p5	TTGAATTCCGCTATTATTTTGGTCT	GCAAGTCGCAAAAGAGATGG	32.0	50.0	57.4	57.7	868 bp	1.0	40.0