Hotspot

atpA

LSC · 10027-11538 · 1512 bp

Back to Gravesia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1512
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	AGCAAAAATGAGATTAAGGATCA	TGTTTGGGGCGATGAAAGAA	30.4	45.0	54.3	58.0	1642 bp	1.0	40.0
atpA_p2	AGCAAAAATGAGATTAAGGATCAA	TGTTTGGGGCGATGAAAGAA	29.2	45.0	55.1	58.0	1642 bp	1.0	40.0
atpA_p3	AGCAAAAATGAGATTAAGGATCA	CTGTTTGGGGCGATGAAAGA	30.4	50.0	54.3	58.5	1643 bp	1.0	40.0
atpA_p4	GCAAAAATGAGATTAAGGATCA	TGTTTGGGGCGATGAAAGAA	31.8	45.0	52.8	58.0	1641 bp	1.0	40.0
atpA_p5	GCAAAAATGAGATTAAGGATCAA	TGTTTGGGGCGATGAAAGAA	30.4	45.0	53.6	58.0	1641 bp	1.0	40.0