Hotspot

rpoB-trnC-GCA

LSC · 27015-28448 · 1434 bp

Back to Gravesia

Pi 0
MarkerSeek score 40.00
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1434
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 TCACTTCCATCCACCAGCAT TTTTGGTTGATCAGGCGACA 50.0 45.0 59.0 58.0 1537 bp 1.0 71.7
rpoB-trnC-GCA_p2 TTCCCTCACTTCCATCCACC TTTTGGTTGATCAGGCGACA 55.0 45.0 59.0 58.0 1542 bp 1.0 71.7
rpoB-trnC-GCA_p3 TCACTTCCATCCACCAGCAT CCGGATTTGAACTGGGGAAA 50.0 50.0 59.0 58.1 1516 bp 1.0 71.3
rpoB-trnC-GCA_p4 TTCCCTCACTTCCATCCACC CCGGATTTGAACTGGGGAAA 55.0 50.0 59.0 58.1 1521 bp 1.0 71.3
rpoB-trnC-GCA_p5 GACATTCCCTCACTTCCATCC TTTTGGTTGATCAGGCGACA 52.4 45.0 58.1 58.0 1546 bp 1.0 71.2