Hotspot

atpH-atpI

LSC · 13723-14688 · 966 bp

Back to Gravesia

Pi 0
MarkerSeek score 40.00
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
966
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 GAAGCAAGTCCGACAGCTAA TAGCCGCCGCTTATATAGGA 50.0 50.0 57.9 57.9 1072 bp 1.0 78.3
atpH-atpI_p2 GAAGCAAGTCCGACAGCTAA TTTTTGCAACTTTAGCCGCC 50.0 45.0 57.9 58.1 1084 bp 1.0 78.2
atpH-atpI_p3 AACAGAAGCGGCAGAAATCA TAGCCGCCGCTTATATAGGA 45.0 50.0 58.1 57.9 1040 bp 1.0 78.1
atpH-atpI_p4 AACAGAAGCGGCAGAAATCA TTTTTGCAACTTTAGCCGCC 45.0 45.0 58.1 58.1 1052 bp 1.0 78.0
atpH-atpI_p5 CAGCAGCAATAACAGAAGCG TAGCCGCCGCTTATATAGGA 50.0 50.0 57.8 57.9 1050 bp 1.0 77.6