Hotspot

rps16-trnQ-UUG

LSC · 5660-6646 · 987 bp

Back to Gravesia

Pi 0
MarkerSeek score 40.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
987
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 1052 bp 1.0 73.1
rps16-trnQ-UUG_p2 GCACGTTGCTTTCTACCACA GAGGTTCGAATCCTTCCGTC 50.0 55.0 59.1 58.1 1055 bp 1.0 70.9
rps16-trnQ-UUG_p3 TAAGAATCCACGGACGATCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 56.8 58.1 1088 bp 1.0 70.0
rps16-trnQ-UUG_p4 CGTTGCTTTCTACCACATCG TTCGGAGGTTCGAATCCTTC 50.0 50.0 57.2 57.3 1056 bp 1.0 68.4
rps16-trnQ-UUG_p5 CGTTGCTTTCTACCACATCG TAAGGCAACGGGTTTTGGTC 50.0 50.0 57.2 58.7 1082 bp 1.0 68.3