primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	TCAAATGATTATTGCTCCCCC	AGAATTCGTGTGCTTGGGAG	21	20	42.9	50.0	56.0	58.2	0.0	0.0	3.226875	309	1370	1191-1191	1.000000	54.2
psbA_p2	psbA	2	TCAAATGATTATTGCTCCCCC	AGAGAATTCGTGTGCTTGGG	21	20	42.9	50.0	56.0	58.2	0.0	0.0	3.226875	309	1370	1193-1193	1.000000	54.2
psbA_p3	psbA	3	TCAAATGATTATTGCTCCCCC	GAGAATTCGTGTGCTTGGGA	21	20	42.9	50.0	56.0	58.2	0.0	0.0	3.231977	309	1370	1192-1192	1.000000	54.1
psbA_p4	psbA	4	TCAAATGATTATTGCTCCCCC	TGGGAGTCCCTGATGATGAA	21	20	42.9	50.0	56.0	57.7	0.0	28.7	3.309184	309	1370	1177-1177	1.000000	53.5
psbA_p5	psbA	5	TCAAATGATTATTGCTCCCCC	TTGGGAGTCCCTGATGATGA	21	20	42.9	50.0	56.0	57.7	0.0	28.7	3.309184	309	1370	1178-1178	1.000000	53.5
matK-trnK-UUU_p1	matK-trnK-UUU	1	AGGAAGTTGTGTTGCCAAGA	AACGGTAGAGTACTCGGCTT	20	20	45.0	50.0	57.6	58.2	0.0	17.1	0.603792	3452	4159	788-788	1.000000	75.2
matK-trnK-UUU_p2	matK-trnK-UUU	2	AGGAAGTTGTGTTGCCAAGA	ACGGTAGAGTACTCGGCTTT	20	20	45.0	50.0	57.6	58.2	0.0	17.1	0.603792	3452	4159	787-787	1.000000	75.2
matK-trnK-UUU_p3	matK-trnK-UUU	3	AGGAAGTTGTGTTGCCAAGA	GGGTTGCTAACTCAACGGTA	20	20	45.0	50.0	57.6	57.5	0.0	0.0	0.896129	3452	4159	801-801	1.000000	72.8
matK-trnK-UUU_p4	matK-trnK-UUU	4	AGGAAGTTGTGTTGCCAAGA	TTCGGGTTGCTAACTCAACG	20	20	45.0	50.0	57.6	58.5	0.0	0.0	0.931013	3452	4159	804-804	1.000000	72.6
matK-trnK-UUU_p5	matK-trnK-UUU	5	AGGAAGTTGTGTTGCCAAGAT	ACGGTAGAGTACTCGGCTTT	21	20	42.9	50.0	58.1	58.2	0.0	17.1	1.218972	3452	4159	787-787	1.000000	70.2
rps16_p1	rps16	1	AAAAAGAGGGAGGGAGAGGG	GTTAAAACCCGGGGGAATGT	20	20	55.0	50.0	58.3	58.1	0.0	27.9	0.397506	4605	5659	1157-1157	1.000000	76.8
rps16_p2	rps16	2	AAAAAGAGGGAGGGAGAGGG	AGTTAAAACCCGGGGGAATG	20	20	55.0	50.0	58.3	57.8	0.0	27.9	0.550375	4605	5659	1158-1158	1.000000	75.6
rps16_p3	rps16	3	AAAAAGAGGGAGGGAGAGGG	CAGTTAAAACCCGGGGGAAT	20	20	55.0	50.0	58.3	57.8	0.0	27.9	0.550375	4605	5659	1159-1159	1.000000	75.6
rps16_p4	rps16	4	AGGGAGAGGGGGATCTACTA	GTTAAAACCCGGGGGAATGT	20	20	55.0	50.0	57.4	58.1	0.0	27.9	0.625940	4605	5659	1147-1147	1.000000	75.0
rps16_p5	rps16	5	AGGGAGAGGGGGATCTACTA	AGTTAAAACCCGGGGGAATG	20	20	55.0	50.0	57.4	57.8	0.0	27.9	0.778809	4605	5659	1148-1148	1.000000	73.8
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.2	58.1	0.0	16.9	0.863824	5660	6646	1052-1052	1.000000	73.1
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GCACGTTGCTTTCTACCACA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	59.1	58.1	0.0	16.9	1.134559	5660	6646	1055-1055	1.000000	70.9
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	TAAGAATCCACGGACGATCC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	56.8	58.1	0.0	16.9	1.247447	5660	6646	1088-1088	1.000000	70.0
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CGTTGCTTTCTACCACATCG	TTCGGAGGTTCGAATCCTTC	20	20	50.0	50.0	57.2	57.3	0.0	16.9	1.455196	5660	6646	1056-1056	1.000000	68.4
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CGTTGCTTTCTACCACATCG	TAAGGCAACGGGTTTTGGTC	20	20	50.0	50.0	57.2	58.7	0.0	0.0	1.467216	5660	6646	1082-1082	1.000000	68.3
atpA_p1	atpA	1	AGCAAAAATGAGATTAAGGATCA	TGTTTGGGGCGATGAAAGAA	23	20	30.4	45.0	54.3	58.0	0.0	0.0	6.699458	10027	11538	1642-1642	1.000000	40.0
atpA_p2	atpA	2	AGCAAAAATGAGATTAAGGATCAA	TGTTTGGGGCGATGAAAGAA	24	20	29.2	45.0	55.1	58.0	0.0	0.0	6.943345	10027	11538	1642-1642	1.000000	40.0
atpA_p3	atpA	3	AGCAAAAATGAGATTAAGGATCA	CTGTTTGGGGCGATGAAAGA	23	20	30.4	50.0	54.3	58.5	0.0	0.0	7.154127	10027	11538	1643-1643	1.000000	40.0
atpA_p4	atpA	4	GCAAAAATGAGATTAAGGATCA	TGTTTGGGGCGATGAAAGAA	22	20	31.8	45.0	52.8	58.0	0.0	0.0	7.205758	10027	11538	1641-1641	1.000000	40.0
atpA_p5	atpA	5	GCAAAAATGAGATTAAGGATCAA	TGTTTGGGGCGATGAAAGAA	23	20	30.4	45.0	53.6	58.0	0.0	0.0	7.363692	10027	11538	1641-1641	1.000000	40.0
atpF_p1	atpF	1	CGTCGGCTTTAATGGTTACC	TGTAAAAGAAAGGGGATTGT	20	20	50.0	35.0	56.9	52.2	0.0	0.0	6.888850	11591	12962	1466-1466	1.000000	40.0
atpF_p2	atpF	2	CGTCGGCTTTAATGGTTACC	ATGTAAAAGAAAGGGGATTGT	20	21	50.0	33.3	56.9	52.9	0.0	0.0	7.157026	11591	12962	1467-1467	1.000000	40.0
atpF_p3	atpF	3	TCGTCGGCTTTAATGGTTACC	TGTAAAAGAAAGGGGATTGT	21	20	47.6	35.0	58.4	52.2	0.0	0.0	7.168877	11591	12962	1467-1467	1.000000	40.0
atpF_p4	atpF	4	TCGTCGGCTTTAATGGTTACC	ATGTAAAAGAAAGGGGATTGT	21	21	47.6	33.3	58.4	52.9	0.0	0.0	7.437052	11591	12962	1468-1468	1.000000	40.0
atpF_p5	atpF	5	CGTCGGCTTTAATGGTTACCA	TGTAAAAGAAAGGGGATTGT	21	20	47.6	35.0	58.7	52.2	6.5	0.0	7.440459	11591	12962	1466-1466	1.000000	40.0
atpH-atpI_p1	atpH-atpI	1	GAAGCAAGTCCGACAGCTAA	TAGCCGCCGCTTATATAGGA	20	20	50.0	50.0	57.9	57.9	16.1	4.5	0.213567	13723	14688	1072-1072	1.000000	78.3
atpH-atpI_p2	atpH-atpI	2	GAAGCAAGTCCGACAGCTAA	TTTTTGCAACTTTAGCCGCC	20	20	50.0	45.0	57.9	58.1	16.1	0.0	0.220903	13723	14688	1084-1084	1.000000	78.2
atpH-atpI_p3	atpH-atpI	3	AACAGAAGCGGCAGAAATCA	TAGCCGCCGCTTATATAGGA	20	20	45.0	50.0	58.1	57.9	1.4	4.5	0.237062	13723	14688	1040-1040	1.000000	78.1
atpH-atpI_p4	atpH-atpI	4	AACAGAAGCGGCAGAAATCA	TTTTTGCAACTTTAGCCGCC	20	20	45.0	45.0	58.1	58.1	1.4	0.0	0.244397	13723	14688	1052-1052	1.000000	78.0
atpH-atpI_p5	atpH-atpI	5	CAGCAGCAATAACAGAAGCG	TAGCCGCCGCTTATATAGGA	20	20	50.0	50.0	57.8	57.9	0.0	4.5	0.302945	13723	14688	1050-1050	1.000000	77.6
atpI_p1	atpI	1	CCAAGTAGATTATCTTGAATTCCGC	GCAAGTCGCAAAAGAGATGG	25	20	40.0	50.0	58.1	57.7	0.0	0.0	5.360702	14689	15432	882-882	1.000000	40.0
atpI_p2	atpI	2	TGAATTCCGCTATTATTTTGGTCT	GCAAGTCGCAAAAGAGATGG	24	20	33.3	50.0	56.7	57.7	2.3	0.0	5.517703	14689	15432	867-867	1.000000	40.0
atpI_p3	atpI	3	ACTGCCTTATTAGTTATCCCT	GCAAGTCGCAAAAGAGATGG	21	20	38.1	50.0	53.4	57.7	0.0	0.0	5.853108	14689	15432	928-928	1.000000	40.0
atpI_p4	atpI	4	TGAATTCCGCTATTATTTTGGTC	GCAAGTCGCAAAAGAGATGG	23	20	34.8	50.0	55.4	57.7	2.3	0.0	5.884695	14689	15432	867-867	1.000000	40.0
atpI_p5	atpI	5	TTGAATTCCGCTATTATTTTGGTCT	GCAAGTCGCAAAAGAGATGG	25	20	32.0	50.0	57.4	57.7	2.3	0.0	5.892505	14689	15432	868-868	1.000000	40.0
rps2_p1	rps2	1	TCCCCACTCATTTTTATCTCA	TTCCGTCGGAACAATTGGTT	21	20	38.1	45.0	54.2	58.0	0.0	22.8	4.814573	15644	16357	902-902	1.000000	41.5
rps2_p2	rps2	2	TCCCCACTCATTTTTATCTCA	CGTCCAATCCACGGTAGAAG	21	20	38.1	55.0	54.2	58.1	0.0	0.0	4.862094	15644	16357	928-928	1.000000	41.1
rps2_p3	rps2	3	TCCCCACTCATTTTTATCTCA	TACGTCCAATCCACGGTAGA	21	20	38.1	50.0	54.2	57.9	0.0	0.0	4.911936	15644	16357	930-930	1.000000	40.7
rps2_p4	rps2	4	TCCCCACTCATTTTTATCTCA	GAAGAAAAGGTTCCGTCGGA	21	20	38.1	50.0	54.2	57.8	0.0	10.7	4.943992	15644	16357	912-912	1.000000	40.4
rps2_p5	rps2	5	TCCCCACTCATTTTTATCTCA	AGAAGAAAAGGTTCCGTCGG	21	20	38.1	50.0	54.2	57.8	0.0	4.4	4.949983	15644	16357	913-913	1.000000	40.4
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	TCACTTCCATCCACCAGCAT	TTTTGGTTGATCAGGCGACA	20	20	50.0	45.0	59.0	58.0	0.0	0.0	1.031488	27015	28448	1537-1537	1.000000	71.7
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	TTCCCTCACTTCCATCCACC	TTTTGGTTGATCAGGCGACA	20	20	55.0	45.0	59.0	58.0	0.0	0.0	1.033815	27015	28448	1542-1542	1.000000	71.7
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TCACTTCCATCCACCAGCAT	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	59.0	58.1	0.0	0.0	1.087881	27015	28448	1516-1516	1.000000	71.3
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TTCCCTCACTTCCATCCACC	CCGGATTTGAACTGGGGAAA	20	20	55.0	50.0	59.0	58.1	0.0	0.0	1.090208	27015	28448	1521-1521	1.000000	71.3
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	GACATTCCCTCACTTCCATCC	TTTTGGTTGATCAGGCGACA	21	20	52.4	45.0	58.1	58.0	0.0	0.0	1.101314	27015	28448	1546-1546	1.000000	71.2
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.088172	28530	29382	926-926	1.000000	79.3
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	CCTTTAGAGTCCACTTCGCC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.093668	28530	29382	985-985	1.000000	79.3
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTCCCCAGTTCAAATCCGG	CTTTAGAGTCCACTTCGCCC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.093668	28530	29382	984-984	1.000000	79.3
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.273256	28530	29382	920-920	1.000000	77.8
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTTCCCCAGTTCAAATCCGG	AGACGACCATTAAAGCAGCC	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.343061	28530	29382	935-935	1.000000	77.3