Hotspot

ycf53

Genome · 52005-52724 · 720 bp

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Pi 0.104

MarkerSeek score 79.40

Primer available yes

Label ycf53

Feature Metadata

label_name: ycf53
feature_type: gene
region: Genome
length_bp: 720
variable_sites: 110
pi: 0.104
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf53_p1	AAATTCATTGACCAGCCCGA	GGTCCAGGAGATCCATCTGT	45.0	55.0	57.8	58.2	1882-1883 bp	1.0	84.7
ycf53_p2	AATTCATTGACCAGCCCGAA	GGTCCAGGAGATCCATCTGT	45.0	55.0	57.8	58.2	1881-1882 bp	1.0	84.7
ycf53_p3	AATTCATTGACCAGCCCGAA	TGGTCCAGGAGATCCATCTG	45.0	55.0	57.8	58.2	1882-1883 bp	1.0	84.7
ycf53_p4	TCCAAGTCGGCAATTCAGTC	GGTCCAGGAGATCCATCTGT	50.0	55.0	58.2	58.2	1698 bp	0.333333	50.2
ycf53_p5	TCCAAGTCGGCAATTCAGTC	TGGTCCAGGAGATCCATCTG	50.0	55.0	58.2	58.2	1699 bp	0.333333	50.2