Hotspot

ycf38

Genome · 110242-111117 · 876 bp

Back to Grateloupia

Pi 0.0909

MarkerSeek score 79.40

Primer available yes

Label ycf38

Feature Metadata

label_name: ycf38
feature_type: gene
region: Genome
length_bp: 876
variable_sites: 119
pi: 0.0909
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf38_p1	CAACAGTGCCAGCTGCTATA	GCTCCTGCACGACAAGAATA	50.0	50.0	58.0	58.0	1942 bp	0.666667	73.7
ycf38_p2	CAGAAATTAAGCGTCCGGGA	GCTCCTGCACGACAAGAATA	50.0	50.0	58.0	58.0	1381 bp	0.333333	53.1
ycf38_p3	GCTAGTATCCCAACAGTGCC	GCTCCTGCACGACAAGAATA	55.0	50.0	58.0	58.0	1952 bp	0.333333	52.9
ycf38_p4	ATGGGTTAAATCCGTCTGGC	GCTCCTGCACGACAAGAATA	50.0	50.0	57.9	58.0	1894 bp	0.333333	52.8
ycf38_p5	CAGAAATTAAGCGTCCGGGA	AACTTGAACAAGCTCCTGCA	50.0	45.0	58.0	57.9	1392 bp	0.333333	52.7