Hotspot

syh

Genome · 80146-81402 · 1257 bp

Back to Grateloupia

Pi 0.0994

MarkerSeek score 79.70

Primer available yes

Label syh

Feature Metadata

label_name: syh
feature_type: gene
region: Genome
length_bp: 1257
variable_sites: 186
pi: 0.0994
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
syh_p1	TCCTTGCCCGATCACAAAAA	ATACTGGGGTGGGAGGATTC	45.0	55.0	58.0	58.2	1461 bp	0.333333	51.8
syh_p2	TCCTTGCCCGATCACAAAAA	TGGCTACACCCCAATAGCTA	45.0	50.0	58.0	57.8	1399 bp	0.333333	51.8
syh_p3	TCCTTGCCCGATCACAAAAA	CTTGGCTACACCCCAATAGC	45.0	55.0	58.0	58.3	1401 bp	0.333333	50.7
syh_p4	TCCTTGCCCGATCACAAAAA	GCTTGGCTACACCCCAATAG	45.0	55.0	58.0	58.3	1402 bp	0.333333	50.7
syh_p5	TGCCATTGGATTAGCACCAG	ATACTGGGGTGGGAGGATTC	50.0	55.0	58.2	58.2	2031 bp	0.333333	50.0