Hotspot

trnG-UCC-pafII

Genome · 186402-186481 · 80 bp

Back to Grateloupia

Pi 0.177

MarkerSeek score 79.70

Primer available yes

Label trnG-UCC-pafII

Feature Metadata

label_name: trnG-UCC-pafII
feature_type: igs
region: Genome
length_bp: 80
variable_sites: 21
pi: 0.177
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-pafII_p1	CCCGCATCATTAGCTTGGAA	AACCAGGCAACATCACTAGC	50.0	50.0	58.3	58.2	192-194 bp	1.0	84.9
trnG-UCC-pafII_p2	CCCGCATCATTAGCTTGGAA	CCAGGCAACATCACTAGCTC	50.0	55.0	58.3	58.3	190-192 bp	1.0	83.6
trnG-UCC-pafII_p3	ATCGAACCCGCATCATTAGC	AACCAGGCAACATCACTAGC	50.0	50.0	58.5	58.2	198-200 bp	1.0	83.5
trnG-UCC-pafII_p4	CCCGCATCATTAGCTTGGAA	CAGGCAACATCACTAGCTCA	50.0	50.0	58.3	57.6	189-191 bp	1.0	83.1
trnG-UCC-pafII_p5	AACCCGCATCATTAGCTTGG	AACCAGGCAACATCACTAGC	50.0	50.0	58.6	58.2	194-196 bp	1.0	82.6