Hotspot

orf12-trnG-UCC

Genome · 185681-186330 · 650 bp

Back to Grateloupia

Pi 0.255

MarkerSeek score 83.10

Primer available yes

Label orf12-trnG-UCC

Feature Metadata

label_name: orf12-trnG-UCC
feature_type: igs
region: Genome
length_bp: 650
variable_sites: 224
pi: 0.255
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf12-trnG-UCC_p1	ATCCTGAAGTTTTTGGGCTT	ACACGTGTTGGACAACCTTT	40.0	45.0	55.4	58.2	1419 bp	0.333333	31.2
orf12-trnG-UCC_p2	ATCCTGAAGTTTTTGGGCTT	AACCAGGCAACATCACTAGC	40.0	50.0	55.4	58.2	1380 bp	0.333333	31.2
orf12-trnG-UCC_p3	ATCCTGAAGTTTTTGGGCTT	TTCCAAGCTAATGATGCGGG	40.0	50.0	55.4	58.3	1206 bp	0.333333	30.0
orf12-trnG-UCC_p4	ATCCTGAAGTTTTTGGGCTT	CCAGGCAACATCACTAGCTC	40.0	55.0	55.4	58.3	1378 bp	0.333333	29.8
orf12-trnG-UCC_p5	ATCCTGAAGTTTTTGGGCTT	CAGGCAACATCACTAGCTCA	40.0	50.0	55.4	57.6	1377 bp	0.333333	29.4