Hotspot

moeB-rpl9

Genome · 93063-94833 · 1771 bp

Back to Grateloupia

Pi 0.194

MarkerSeek score 57.10

Primer available yes

Label moeB-rpl9

Feature Metadata

label_name: moeB-rpl9
feature_type: igs
region: Genome
length_bp: 1771
variable_sites: 39
pi: 0.194
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.08
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
moeB-rpl9_p1	ACCGGGAATAATTGGCGTTT	CTTGCCTTCTTGACCCAGTT	45.0	50.0	58.2	58.0	2363 bp	0.333333	52.0
moeB-rpl9_p2	AAGGTGGTCCGAGGTATTCT	CTTGCCTTCTTGACCCAGTT	50.0	50.0	57.8	58.0	2451 bp	0.333333	51.2
moeB-rpl9_p3	ACCGGGAATAATTGGCGTTT	TTCTTGACCCAGTTTTGGCA	45.0	45.0	58.2	57.8	2357 bp	0.333333	50.9
moeB-rpl9_p4	GCAGCTCTAATTCTCGTGCA	CTTGCCTTCTTGACCCAGTT	50.0	50.0	58.4	58.0	1980 bp	0.333333	50.4
moeB-rpl9_p5	TGCAGCTCTAATTCTCGTGC	CTTGCCTTCTTGACCCAGTT	50.0	50.0	58.4	58.0	1981 bp	0.333333	50.4