Hotspot

rpl11-trnW-CCA

Genome · 90426-91766 · 1341 bp

Back to Grateloupia

Pi 0.267

MarkerSeek score 83.30

Primer available yes

Label rpl11-trnW-CCA

Feature Metadata

label_name: rpl11-trnW-CCA
feature_type: igs
region: Genome
length_bp: 1341
variable_sites: 492
pi: 0.267
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl11-trnW-CCA_p1	GCGAGCTGTTCCAGATACAA	TGCAGAAGACGCTAATCCAC	50.0	50.0	58.0	58.0	2058-2834 bp	0.666667	78.5
rpl11-trnW-CCA_p2	GCGAGCTGTTCCAGATACAA	GATGTCGAGGGTTCAAGTCC	50.0	55.0	58.0	58.0	1771-2560 bp	0.666667	78.5
rpl11-trnW-CCA_p3	AGGCTGTCCTGAACCTTTTG	TGCAGAAGACGCTAATCCAC	50.0	50.0	58.0	58.0	1935 bp	0.333333	53.1
rpl11-trnW-CCA_p4	AGGCTGTCCTGAACCTTTTG	GATGTCGAGGGTTCAAGTCC	50.0	55.0	58.0	58.0	1648 bp	0.333333	53.1
rpl11-trnW-CCA_p5	CGTGTTAAGATCCTGCAGCT	TGCAGAAGACGCTAATCCAC	50.0	50.0	58.0	58.0	2013 bp	0.333333	53.1