Hotspot

cpeA-ccsA

Genome · 12451-13020 · 570 bp

Back to Grateloupia

Pi 0.224

MarkerSeek score 82.90

Primer available yes

Label cpeA-ccsA

Feature Metadata

label_name: cpeA-ccsA
feature_type: igs
region: Genome
length_bp: 570
variable_sites: 174
pi: 0.224
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cpeA-ccsA_p1	CGGCGGAAAAACTAGCTAGT	AATGGACGGCAGTTAATCCC	50.0	50.0	58.0	57.9	1206 bp	0.333333	52.9
cpeA-ccsA_p2	CGGCGGAAAAACTAGCTAGT	CCCATACAGCTCCAGCAATT	50.0	50.0	58.0	57.9	1671 bp	0.333333	52.8
cpeA-ccsA_p3	GCGCCTAATCAACTACTGCT	AATGGACGGCAGTTAATCCC	50.0	50.0	58.1	57.9	1055 bp	0.333333	52.4
cpeA-ccsA_p4	GCGATAGAGTTTCTGCTGCT	AATGGACGGCAGTTAATCCC	50.0	50.0	58.1	57.9	1470 bp	0.333333	52.4
cpeA-ccsA_p5	GCGCCTAATCAACTACTGCT	CCCATACAGCTCCAGCAATT	50.0	50.0	58.1	57.9	1520 bp	0.333333	52.4