Hotspot

ycf80-trnR-CCG

Genome · 7066-8032 · 967 bp

Back to Grateloupia

Pi 0.144

MarkerSeek score 80.50

Primer available yes

Label ycf80-trnR-CCG

Feature Metadata

label_name: ycf80-trnR-CCG
feature_type: igs
region: Genome
length_bp: 967
variable_sites: 202
pi: 0.144
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf80-trnR-CCG_p1	AAATGTCTGTGTGGCTGTGT	CGGTCGAAGGTTCAAATCCT	45.0	50.0	57.9	57.9	1204 bp	0.333333	52.1
ycf80-trnR-CCG_p2	AAATGTCTGTGTGGCTGTGT	TTGGGCATGTAACTCAGTGG	45.0	50.0	57.9	57.8	1249 bp	0.333333	51.3
ycf80-trnR-CCG_p3	AAATGTCTGTGTGGCTGTGT	AGTTCGTTCATTGCAGGAGT	45.0	45.0	57.9	57.4	1900 bp	0.333333	48.5
ycf80-trnR-CCG_p4	AAATGTCTGTGTGGCTGTGT	TGGGCATGTAACTCAGTGGA	45.0	50.0	57.9	58.6	1248 bp	0.333333	47.7
ycf80-trnR-CCG_p5	AAATGTCTGTGTGGCTGTGT	GGGCATGTAACTCAGTGGAT	45.0	50.0	57.9	57.3	1247 bp	0.333333	47.1