Hotspot

rps16

LSC · 4753-5824 · 1072 bp

Back to Graptopetalum

Pi 0.015

MarkerSeek score 54.60

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1072
variable_sites: 16
pi: 0.015
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GAGTTAGCAACCCCAAACCA	CGGGACTCAAAACTGTGGAT	50.0	50.0	58.0	57.8	2003-2014 bp	1.0	80.0
rps16_p2	TGAGTTAGCAACCCCAAACC	CGGGACTCAAAACTGTGGAT	50.0	50.0	58.0	57.8	2004-2015 bp	1.0	80.0
rps16_p3	GAGTTAGCAACCCCAAACCA	AGTTGAATTCTTCGTGCGGA	50.0	45.0	58.0	57.8	1907-1913 bp	1.0	79.9
rps16_p4	TGAGTTAGCAACCCCAAACC	AGTTGAATTCTTCGTGCGGA	50.0	45.0	58.0	57.8	1908-1914 bp	1.0	79.9
rps16_p5	GAGTTAGCAACCCCAAACCA	ACGAATTTCACCACGTACCC	50.0	50.0	58.0	58.2	1882 bp	0.5	58.3