Hotspot

atpH

LSC · 13598-13843 · 246 bp

Back to Graptopetalum

Pi 0.00813

MarkerSeek score 45.40

Primer available yes

Label atpH

Feature Metadata

label_name: atpH
feature_type: gene
region: LSC
length_bp: 246
variable_sites: 2
pi: 0.00813
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH_p1	TGTAGTTTTTAGTTAGATTTCTAGGA	TTTTGGTGCGAGGAACTTCT	26.9	45.0	53.9	57.7	298 bp	1.0	40.5
atpH_p2	TGTAGTTTTTAGTTAGATTTCTAGG	TTTTGGTGCGAGGAACTTCT	28.0	45.0	52.6	57.7	298 bp	1.0	40.5
atpH_p3	ATGTAGTTTTTAGTTAGATTTCTAGGA	TTTTGGTGCGAGGAACTTCT	25.9	45.0	54.4	57.7	299 bp	1.0	40.5
atpH_p4	TGTAGTTTTTAGTTAGATTTCTAGGAT	TTTTGGTGCGAGGAACTTCT	25.9	45.0	54.4	57.7	298 bp	1.0	40.5
atpH_p5	GTAGTTTTTAGTTAGATTTCTAGGA	TTTTGGTGCGAGGAACTTCT	28.0	45.0	52.3	57.7	297 bp	1.0	40.5