Hotspot

ndhE-ndhG

SSC · 115086-115264 · 179 bp

Back to Graptopetalum

Pi 0.0114

MarkerSeek score 61.20

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 179
variable_sites: 2
pi: 0.0114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	ATTCCGGCTCGTGATCAATC	AATTGCGGTAGCTCGTCAAT	50.0	45.0	58.1	58.0	275-279 bp	1.0	80.5
ndhE-ndhG_p2	GGCTCTAACCATATTCCGGC	AATTGCGGTAGCTCGTCAAT	55.0	45.0	58.2	58.0	287-291 bp	1.0	79.9
ndhE-ndhG_p3	CCATATTCCGGCTCGTGATC	AATTGCGGTAGCTCGTCAAT	55.0	45.0	58.3	58.0	279-283 bp	1.0	79.2
ndhE-ndhG_p4	ATTCCGGCTCGTGATCAATC	GCTTTGATAGGGGCAATTGC	50.0	50.0	58.1	57.8	289-293 bp	1.0	78.8
ndhE-ndhG_p5	GGCTCTAACCATATTCCGGC	GCTTTGATAGGGGCAATTGC	55.0	50.0	58.2	57.8	301-305 bp	1.0	78.2