Hotspot

ndhD-psaC

SSC · 114132-114263 · 132 bp

Back to Graptopetalum

Pi 0.159

MarkerSeek score 77.50

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 132
variable_sites: 21
pi: 0.159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TGCCTTTTTGAGGGAGAAAGA	AACAACTCGTAGCATGGGTC	42.9	50.0	57.4	57.9	248-251 bp	1.0	73.2
ndhD-psaC_p2	TGCCTTTTTGAGGGAGAAAGA	TCGTAGCATGGGTCTAGCTT	42.9	50.0	57.4	58.2	242-245 bp	1.0	72.4
ndhD-psaC_p3	TGCCTTTTTGAGGGAGAAAGA	GGCATGAAACAACTCGTAGC	42.9	50.0	57.4	57.5	255-258 bp	1.0	69.6
ndhD-psaC_p4	TGCCTTTTTGAGGGAGAAAGA	AAACAACTCGTAGCATGGGT	42.9	45.0	57.4	57.4	249-252 bp	1.0	69.5
ndhD-psaC_p5	TGCCTTTTTGAGGGAGAAAGAA	AACAACTCGTAGCATGGGTC	40.9	50.0	58.1	57.9	248-251 bp	1.0	69.3