Hotspot

rpl36-infA

LSC · 77513-77632 · 120 bp

Back to Graptopetalum

Pi 0.0167

MarkerSeek score 62.10

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 120
variable_sites: 2
pi: 0.0167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	CTATAATTCGTCCTCGCCGG	ACCCGGGGATAGAGTCAAAA	55.0	50.0	58.0	58.0	296 bp	1.0	80.1
rpl36-infA_p2	TTGCTTATGTCTCGGGTTGG	ACCCGGGGATAGAGTCAAAA	50.0	50.0	57.9	58.0	325 bp	1.0	79.3
rpl36-infA_p3	CTATAATTCGTCCTCGCCGG	CTACCCGGGGATAGAGTCAA	55.0	55.0	58.0	57.6	298 bp	1.0	77.4
rpl36-infA_p4	GCTTATGTCTCGGGTTGGAA	ACCCGGGGATAGAGTCAAAA	50.0	50.0	57.6	58.0	323 bp	1.0	77.0
rpl36-infA_p5	TTGCTTATGTCTCGGGTTGG	CTACCCGGGGATAGAGTCAA	50.0	55.0	57.9	57.6	327 bp	1.0	76.6