Hotspot

trnC-GCA-petN

LSC · 28008-28857 · 850 bp

Back to Graptopetalum

Pi 0.0141

MarkerSeek score 61.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 850
variable_sites: 12
pi: 0.0141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	915-919 bp	1.0	78.9
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TTAAACCAGCCCAAGCAAGA	50.0	45.0	58.1	57.6	921-925 bp	1.0	77.4
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	TAAACCAGCCCAAGCAAGAC	50.0	50.0	58.1	58.4	920-924 bp	1.0	77.3
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TTAGAGTCCACTTCGTCCCC	50.0	55.0	58.1	58.4	977-981 bp	1.0	76.8
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.4	57.8	933-937 bp	1.0	76.7