Hotspot

rps16-trnQ-UUG

LSC · 5825-7235 · 1411 bp

Back to Graptopetalum

Pi 0.013

MarkerSeek score 68.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1411
variable_sites: 18
pi: 0.013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1483-1484 bp	1.0	80.7
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1494-1495 bp	1.0	80.2
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	ATAAATCGAAGTGGGGCGTG	45.0	50.0	58.1	58.3	1542-1543 bp	1.0	78.5
rps16-trnQ-UUG_p4	CCACAGCTGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	1504-1505 bp	1.0	78.5
rps16-trnQ-UUG_p5	TCCACAGCTGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	1505-1506 bp	1.0	78.4