Hotspot

trnH-GUG-psbA

LSC · 73-231 · 159 bp

Back to Graptopetalum

Pi 0.0252

MarkerSeek score 64.90

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 159
variable_sites: 4
pi: 0.0252
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	GTAACGCTCACAACTTCCCT	50.0	50.0	57.9	57.8	250-252 bp	1.0	78.9
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	GTAACGCTCACAACTTCCCT	50.0	50.0	58.2	57.8	251-253 bp	1.0	78.7
trnH-GUG-psbA_p3	CTGCCTTAATCCACTTGGCT	TGAACGTAACGCTCACAACT	50.0	45.0	57.9	57.8	255-257 bp	1.0	78.5
trnH-GUG-psbA_p4	ACTGCCTTAATCCACTTGGC	TGAACGTAACGCTCACAACT	50.0	45.0	58.2	57.8	256-258 bp	1.0	78.3
trnH-GUG-psbA_p5	CTGCCTTAATCCACTTGGCT	ATGCATGAACGTAACGCTCA	50.0	45.0	57.9	58.3	260-262 bp	1.0	77.9