Hotspot

ycf91

Genome · 58521-58778 · 258 bp

Back to Gracilariopsis

Pi 0.128

MarkerSeek score 72.50

Primer available yes

Label ycf91

Feature Metadata

label_name: ycf91
feature_type: gene
region: Genome
length_bp: 258
variable_sites: 48
pi: 0.128
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf91_p1	CCACCTGGTCTTAGAAGCTC	CCCTCGACTTTCAGAATCGG	55.0	55.0	57.3	58.1	874 bp	0.333333	47.3
ycf91_p2	CCACCTGGTCTTAGAAGCTC	GAAGGACTTGAACCCTCGAC	55.0	55.0	57.3	57.9	886 bp	0.333333	47.2
ycf91_p3	CCACCTGGTCTTAGAAGCTC	AGAAACCTAGCAGTCCCGAT	55.0	50.0	57.3	58.1	1166 bp	0.333333	46.8
ycf91_p4	CCACCTGGTCTTAGAAGCTC	ACCTAGCAGTCCCGATAACA	55.0	50.0	57.3	57.8	1162 bp	0.333333	46.6
ycf91_p5	CCACCTGGTCTTAGAAGCTC	AGCATGGAAGGACTTGAACC	55.0	50.0	57.3	57.8	892 bp	0.333333	46.2