Hotspot

petF

Genome · 143979-144275 · 297 bp

Back to Gracilariopsis

Pi 0.224

MarkerSeek score 73.70

Primer available yes

Label petF

Feature Metadata

label_name: petF
feature_type: gene
region: Genome
length_bp: 297
variable_sites: 96
pi: 0.224
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petF_p1	GCTGCAGAAATGGTAATGCC	ACCGGAATTTATTGCTCCTGT	50.0	42.9	57.8	57.6	1431-1438 bp	0.666667	59.0
petF_p2	GGATCAGCTGCAGAAATGGT	ACCGGAATTTATTGCTCCTGT	50.0	42.9	58.2	57.6	1437-1444 bp	0.666667	58.8
petF_p3	GGATGATGGATCAGCTGCAG	ACCGGAATTTATTGCTCCTGT	55.0	42.9	58.5	57.6	1444-1451 bp	0.666667	56.9
petF_p4	TGGTAATGCCTGGAGATCGT	ACCGGAATTTATTGCTCCTGT	50.0	42.9	58.5	57.6	1421-1428 bp	0.666667	56.6
petF_p5	TGGATCAGCTGCAGAAATGG	ACCGGAATTTATTGCTCCTGT	50.0	42.9	58.2	57.6	1445 bp	0.333333	40.5