Hotspot

acpP

Genome · 117634-117897 · 264 bp

Back to Gracilariopsis

Pi 0.21

MarkerSeek score 72.50

Primer available yes

Label acpP

Feature Metadata

label_name: acpP
feature_type: gene
region: Genome
length_bp: 264
variable_sites: 78
pi: 0.21
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
acpP_p1	GGAGAGGTGGTAGAGTTGGT	TCGTAGTTGTGGTGAAGAGC	55.0	50.0	58.1	57.6	536-564 bp	1.0	88.0
acpP_p2	CATCTCCAACTTTCGGTGGT	TCGTAGTTGTGGTGAAGAGC	50.0	50.0	57.8	57.6	1054-1081 bp	0.666667	68.0
acpP_p3	CAGAAGTGGTGTCGGCAATA	TCGTAGTTGTGGTGAAGAGC	50.0	50.0	57.9	57.6	774 bp	0.333333	49.1
acpP_p4	AACTTTCGGTGGTAGCACAG	TCGTAGTTGTGGTGAAGAGC	50.0	50.0	58.1	57.6	1074 bp	0.333333	48.9
acpP_p5	GAATCCCACCCTCTCCTCAT	TCGTAGTTGTGGTGAAGAGC	55.0	50.0	58.2	57.6	495 bp	0.333333	48.4