Hotspot

psbD-ompR

Genome · 178707-179229 · 523 bp

Back to Gracilariopsis

Pi 0.126

MarkerSeek score 81.80

Primer available yes

Label psbD-ompR

Feature Metadata

label_name: psbD-ompR
feature_type: igs
region: Genome
length_bp: 523
variable_sites: 89
pi: 0.126
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-ompR_p1	GCACATAACAATGCACCACC	AGATGGTTATGGCGTGTGTC	50.0	50.0	58.0	58.0	1699 bp	0.333333	53.1
psbD-ompR_p2	GGCACATAACAATGCACCAC	AGATGGTTATGGCGTGTGTC	50.0	50.0	58.0	58.0	1700 bp	0.333333	53.1
psbD-ompR_p3	CAATCCAGACCAACCCACAA	AGATGGTTATGGCGTGTGTC	50.0	50.0	58.0	58.0	1169 bp	0.333333	53.1
psbD-ompR_p4	ACAAAAACAGCAATTGGCCC	AGATGGTTATGGCGTGTGTC	45.0	50.0	58.0	58.0	1525 bp	0.333333	52.9
psbD-ompR_p5	TCTGCTGCATCACCATCTTC	AGATGGTTATGGCGTGTGTC	50.0	50.0	58.0	58.0	1753 bp	0.333333	52.9