Hotspot

rpl11-trnW(cca)

Genome · 168460-168709 · 250 bp

Back to Gracilariopsis

Pi 0.163
MarkerSeek score 79.80
Primer available yes
Label rpl11-trnW(cca)

Feature Metadata

label_name
rpl11-trnW(cca)
feature_type
igs
region
Genome
length_bp
250
variable_sites
57
pi
0.163
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl11-trnW(cca)_p1 CTACTGGAGGAGCTGGAGTT GTCGAGGGTTCAAGTCCTTC 55.0 55.0 58.1 57.9 337-351 bp 1.0 89.9
rpl11-trnW(cca)_p2 TTTGGTTGTCCTGATCCAGC GTCGAGGGTTCAAGTCCTTC 50.0 55.0 58.1 57.9 542-556 bp 1.0 88.9
rpl11-trnW(cca)_p3 TTTGGTTGTCCTGATCCAGC GGCAGAACGTAGGTTTCCAA 50.0 50.0 58.1 58.1 570-584 bp 1.0 88.6
rpl11-trnW(cca)_p4 TTTGGTTGTCCTGATCCAGC TTGGCAGAACGTAGGTTTCC 50.0 50.0 58.1 58.1 572-586 bp 1.0 88.6
rpl11-trnW(cca)_p5 TTTGGTTGTCCTGATCCAGC CAGTTGGCAGAACGTAGGTT 50.0 50.0 58.1 58.1 575-589 bp 1.0 88.5