Hotspot

preA-rpl20

Genome · 111611-111996 · 386 bp

Back to Gracilariopsis

Pi 0.204

MarkerSeek score 79.00

Primer available yes

Label preA-rpl20

Feature Metadata

label_name: preA-rpl20
feature_type: igs
region: Genome
length_bp: 386
variable_sites: 108
pi: 0.204
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
preA-rpl20_p1	TGGAAAACCAGCAGGATCAG	AATGCTGCTGTTCGTCCTTA	50.0	45.0	57.8	57.5	789-837 bp	1.0	86.6
preA-rpl20_p2	TGGAAAACCAGCAGGATCAG	TGCTGCTGTTCGTCCTTATG	50.0	50.0	57.8	58.3	832-835 bp	0.666667	69.2
preA-rpl20_p3	CTCTTGGAAAACCAGCAGGA	TGCTGCTGTTCGTCCTTATG	50.0	50.0	57.7	58.3	839 bp	0.333333	48.9
preA-rpl20_p4	TCTCTTGGAAAACCAGCAGG	TGCTGCTGTTCGTCCTTATG	50.0	50.0	57.7	58.3	840 bp	0.333333	48.9
preA-rpl20_p5	CGCCGCAATGTTAAGTGAAA	TGCTGCTGTTCGTCCTTATG	45.0	50.0	57.7	58.3	961 bp	0.333333	48.5