Hotspot

orf461-trnM

Genome · 71288-71869 · 582 bp

Back to Gracilariopsis

Pi 0.0756

MarkerSeek score 82.90

Primer available yes

Label orf461-trnM

Feature Metadata

label_name: orf461-trnM
feature_type: igs
region: Genome
length_bp: 582
variable_sites: 63
pi: 0.0756
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf461-trnM_p1	CCAAGGCAAATTTTTCCACTGA	GGATTGCCAGAAAGTCGAGA	40.9	50.0	57.9	57.6	860-862 bp	0.666667	50.1
orf461-trnM_p2	CCAAGGCAAATTTTTCCACTGA	TTCTTAAGCATCTGTGGCCG	40.9	50.0	57.9	58.3	677 bp	0.333333	34.7
orf461-trnM_p3	TCCAAGGCAAATTTTTCCACTG	TTCTTAAGCATCTGTGGCCG	40.9	50.0	57.9	58.3	678 bp	0.333333	34.7
orf461-trnM_p4	ACTCCAAGGCAAATTTTTCCAC	TTCTTAAGCATCTGTGGCCG	40.9	50.0	57.9	58.3	680 bp	0.333333	34.7
orf461-trnM_p5	TCTAGCATTCCACTCAGATCCT	TTCTTAAGCATCTGTGGCCG	45.5	50.0	58.1	58.3	1016 bp	0.333333	34.6