Hotspot

trnG(tcc)-trnM

Genome · 69729-69783 · 55 bp

Back to Gracilariopsis

Pi 0.127

MarkerSeek score 80.30

Primer available yes

Label trnG(tcc)-trnM

Feature Metadata

label_name: trnG(tcc)-trnM
feature_type: igs
region: Genome
length_bp: 55
variable_sites: 10
pi: 0.127
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG(tcc)-trnM_p1	TTCCAAGCTAATGATGCGGG	GGTTCGAATCCAGCCAGATG	50.0	55.0	58.3	58.7	113-117 bp	1.0	82.2
trnG(tcc)-trnM_p2	GCTAATGATGCGGGTTCGAT	GGTTCGAATCCAGCCAGATG	50.0	55.0	58.5	58.7	107-111 bp	1.0	81.5
trnG(tcc)-trnM_p3	CCAAGCTAATGATGCGGGTT	GGTTCGAATCCAGCCAGATG	50.0	55.0	58.6	58.7	111-115 bp	1.0	80.1
trnG(tcc)-trnM_p4	AATGATGCGGGTTCGATTCC	GGTTCGAATCCAGCCAGATG	50.0	55.0	58.7	58.7	104-108 bp	1.0	80.1
trnG(tcc)-trnM_p5	AAGCTAATGATGCGGGTTCG	GGTTCGAATCCAGCCAGATG	50.0	55.0	58.7	58.7	109-113 bp	1.0	79.6