Hotspot

trnR(ccg)-orf441

Genome · 59071-59876 · 806 bp

Back to Gracilariopsis

Pi 0.118
MarkerSeek score 83.60
Primer available yes
Label trnR(ccg)-orf441

Feature Metadata

label_name
trnR(ccg)-orf441
feature_type
igs
region
Genome
length_bp
806
variable_sites
125
pi
0.118
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR(ccg)-orf441_p1 CCGATTCTGAAAGTCGAGGG TCTCTATCCTGCCAGCAAGT 55.0 50.0 58.1 58.1 1001-1126 bp 0.666667 71.2
trnR(ccg)-orf441_p2 GTCGAGGGTTCAAGTCCTTC TCTCTATCCTGCCAGCAAGT 55.0 50.0 57.9 58.1 989-1114 bp 0.666667 71.1
trnR(ccg)-orf441_p3 CCGATTCTGAAAGTCGAGGG GTTGTCTCTATCCTGCCAGC 55.0 55.0 58.1 58.1 1130 bp 0.333333 52.3
trnR(ccg)-orf441_p4 GTCGAGGGTTCAAGTCCTTC GTTGTCTCTATCCTGCCAGC 55.0 55.0 57.9 58.1 1118 bp 0.333333 52.3
trnR(ccg)-orf441_p5 GGTTCAAGTCCTTCCATGCT GTTGTCTCTATCCTGCCAGC 50.0 55.0 57.8 58.1 1112 bp 0.333333 51.3