Hotspot

gltB-psaC

Genome · 49886-50417 · 532 bp

Back to Gracilariopsis

Pi 0.213

MarkerSeek score 83.10

Primer available yes

Label gltB-psaC

Feature Metadata

label_name: gltB-psaC
feature_type: igs
region: Genome
length_bp: 532
variable_sites: 157
pi: 0.213
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
gltB-psaC_p1	GGGAGGGATTGTCGTTGTTT	GTTTCACAGCGCTTACAACC	50.0	50.0	58.1	58.0	1036 bp	0.333333	52.3
gltB-psaC_p2	ACGGATAGAACTGTTGGTGC	GTTTCACAGCGCTTACAACC	50.0	50.0	57.9	58.0	1428 bp	0.333333	52.3
gltB-psaC_p3	TGGTGCTAGGATATCGGGTT	GTTTCACAGCGCTTACAACC	50.0	50.0	57.9	58.0	1414 bp	0.333333	52.3
gltB-psaC_p4	TTGGTGCTAGGATATCGGGT	GTTTCACAGCGCTTACAACC	50.0	50.0	57.9	58.0	1415 bp	0.333333	52.3
gltB-psaC_p5	GGGAGGGATTGTCGTTGTTT	TTTCACAGCGCTTACAACCT	50.0	45.0	58.1	58.0	1035 bp	0.333333	52.3