Hotspot

trnN(gtt)-trnL(tag)

Genome · 10379-10533 · 155 bp

Back to Gracilariopsis

Pi 0.211

MarkerSeek score 80.50

Primer available yes

Label trnN(gtt)-trnL(tag)

Feature Metadata

label_name: trnN(gtt)-trnL(tag)
feature_type: igs
region: Genome
length_bp: 155
variable_sites: 46
pi: 0.211
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN(gtt)-trnL(tag)_p1	CTCTGCCACTGAGCTACTGA	AGCGTGTCTACCAATTCCAC	55.0	50.0	58.8	57.9	205-208 bp	1.0	83.0
trnN(gtt)-trnL(tag)_p2	TCTGCCACTGAGCTACTGAG	AGCGTGTCTACCAATTCCAC	55.0	50.0	58.8	57.9	204-207 bp	1.0	83.0
trnN(gtt)-trnL(tag)_p3	CTCTGCCACTGAGCTACTGA	TACCAATTCCACCATGTCCG	55.0	50.0	58.8	57.6	197-200 bp	1.0	80.6
trnN(gtt)-trnL(tag)_p4	TCTGCCACTGAGCTACTGAG	TACCAATTCCACCATGTCCG	55.0	50.0	58.8	57.6	196-199 bp	1.0	80.6
trnN(gtt)-trnL(tag)_p5	ACCAATCGGTTAACAGCCG	AGCGTGTCTACCAATTCCAC	52.6	50.0	58.2	57.9	228-231 bp	1.0	80.1