Hotspot

ycf60

Genome · 5597-6211 · 615 bp

Back to Gracilaria

Pi 0.081

MarkerSeek score 80.70

Primer available yes

Label ycf60

Feature Metadata

label_name: ycf60
feature_type: gene
region: Genome
length_bp: 615
variable_sites: 165
pi: 0.081
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf60_p1	AGGGAAGCTTTTTGGGAAGG	GTAAACCTTGCCCAGAACCA	50.0	50.0	58.0	58.0	2025-2120 bp	0.294118	63.6
ycf60_p2	AGGGAAGCTTTTTGGGAAGG	TGTAAACCTTGCCCAGAACC	50.0	50.0	58.0	58.0	2026-2121 bp	0.294118	63.6
ycf60_p3	AGGGAAGCTTTTTGGGAAGG	GCCCAGAACCAATTGGAGAA	50.0	50.0	58.0	58.1	2016-2111 bp	0.235294	60.7
ycf60_p4	GGTTTCTCCGCTTAAACCCA	GTAAACCTTGCCCAGAACCA	50.0	50.0	58.1	58.0	1626-1631 bp	0.117647	46.0
ycf60_p5	GGTTTCTCCGCTTAAACCCA	TGTAAACCTTGCCCAGAACC	50.0	50.0	58.1	58.0	1627-1632 bp	0.117647	46.0