Hotspot

ycf23

Genome · 50156-50962 · 807 bp

Back to Gracilaria

Pi 0.171

MarkerSeek score 83.00

Primer available yes

Label ycf23

Feature Metadata

label_name: ycf23
feature_type: gene
region: Genome
length_bp: 807
variable_sites: 436
pi: 0.171
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf23_p1	GCAAAGCTGGTCAAATTGCA	TTCTTGTTGCAGCTCTCACT	45.0	45.0	58.1	57.4	1667-1681 bp	0.294118	54.6
ycf23_p2	TGCAAAGCTGGTCAAATTGC	TTCTTGTTGCAGCTCTCACT	45.0	45.0	58.1	57.4	1668-1682 bp	0.294118	54.6
ycf23_p3	AGAAATGGTTCCTTGGGACG	TTCTTGTTGCAGCTCTCACT	50.0	45.0	57.8	57.4	1690-1704 bp	0.235294	51.6
ycf23_p4	ACGTAGTATGGGGCTAGCAT	TTCTTGTTGCAGCTCTCACT	50.0	45.0	58.0	57.4	1552-1560 bp	0.117647	48.5
ycf23_p5	AGCGTGTCCAACAGACTTTT	TTCTTGTTGCAGCTCTCACT	45.0	45.0	58.0	57.4	1600-1614 bp	0.117647	48.2