Hotspot

petF

Genome · 142305-142598 · 294 bp

Back to Gracilaria

Pi 0.233

MarkerSeek score 75.00

Primer available yes

Label petF

Feature Metadata

label_name: petF
feature_type: gene
region: Genome
length_bp: 294
variable_sites: 169
pi: 0.233
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petF_p1	TACAGCCCGTAGAACAAACG	GCTCCGAATAGAAGAAGCCA	50.0	50.0	57.9	57.4	994-1003 bp	0.117647	48.4
petF_p2	TACAGCCCGTAGAACAAACG	TGCTCCGAATAGAAGAAGCC	50.0	50.0	57.9	57.4	995-1004 bp	0.117647	48.4
petF_p3	CGAAAGAAGAAGGCGGAAGA	GCTCCGAATAGAAGAAGCCA	50.0	50.0	57.9	57.4	1368 bp	0.117647	44.0
petF_p4	CGAAAGAAGAAGGCGGAAGA	TGCTCCGAATAGAAGAAGCC	50.0	50.0	57.9	57.4	1369 bp	0.117647	44.0
petF_p5	GCAAAACTGTAGGAGCTGGT	GCTCCGAATAGAAGAAGCCA	50.0	50.0	58.1	57.4	1149 bp	0.058824	36.7