Hotspot

orf241

Genome · 76215-76940 · 726 bp

Back to Gracilaria

Pi 0.176

MarkerSeek score 84.90

Primer available yes

Label orf241

Feature Metadata

label_name: orf241
feature_type: gene
region: Genome
length_bp: 726
variable_sites: 410
pi: 0.176
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf241_p1	TGCGTATACCAATTTCGCCA	AACACCAACTTCAGCAAGCT	45.0	45.0	58.0	58.2	1334-1361 bp	0.647059	73.6
orf241_p2	TGCGTATACCAATTTCGCCA	TGGACAATGGGTGTTTGGAG	45.0	50.0	58.0	58.0	1507 bp	0.117647	48.0
orf241_p3	CCTTCATCTAAAGCGCGTCT	TGGACAATGGGTGTTTGGAG	50.0	50.0	58.1	58.0	1668 bp	0.117647	47.4
orf241_p4	AACCTTCATCTAAAGCGCGT	TGGACAATGGGTGTTTGGAG	45.0	50.0	57.9	58.0	1670 bp	0.117647	47.2
orf241_p5	ATTGTGCTCCCCACTCTTTC	TGGACAATGGGTGTTTGGAG	50.0	50.0	57.8	58.0	1736 bp	0.058824	40.7