Hotspot

ompR

Genome · 178346-179074 · 729 bp

Back to Gracilaria

Pi 0.0896

MarkerSeek score 83.10

Primer available yes

Label ompR

Feature Metadata

label_name: ompR
feature_type: gene
region: Genome
length_bp: 729
variable_sites: 213
pi: 0.0896
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ompR_p1	ACAAGGTTTGTTGGTTCTGGA	AGGATATTGTCAAGACCTCA	42.9	40.0	57.9	53.0	1689 bp	0.058824	2.4
ompR_p2	ACAAGGTTTGTTGGTTCTGGA	AGGATATTGTCAAGACCTCAT	42.9	38.1	57.9	53.7	1689 bp	0.058824	2.4
ompR_p3	AGGTTTGTTGGTTCTGGAGA	AGGATATTGTCAAGACCTCA	45.0	40.0	56.6	53.0	1686 bp	0.058824	2.4
ompR_p4	ACAAGGTTTGTTGGTTCTGGA	AGGATATTGTCAAGACCTCATAGT	42.9	37.5	57.9	56.7	1689 bp	0.058824	2.4
ompR_p5	ACAAGGTTTGTTGGTTCTGG	AGGATATTGTCAAGACCTCA	45.0	40.0	56.4	53.0	1689 bp	0.058824	2.4