Hotspot

dsbD

Genome · 9550-10284 · 735 bp

Back to Gracilaria

Pi 0.167

MarkerSeek score 82.30

Primer available yes

Label dsbD

Feature Metadata

label_name: dsbD
feature_type: gene
region: Genome
length_bp: 735
variable_sites: 372
pi: 0.167
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dsbD_p1	AATAGGAGCAATTCGGCCAG	AGCGTGTCTACCAATTCCAC	50.0	50.0	58.0	57.9	1603-1658 bp	0.941176	87.2
dsbD_p2	AATAGGAGCAATTCGGCCAG	TCAAATCCTTCCTGGGGAGT	50.0	50.0	58.0	57.9	1373-1400 bp	0.941176	87.1
dsbD_p3	AATAGGAGCAATTCGGCCAG	TACATGCGGACAGAGAGACT	50.0	50.0	58.0	57.9	1661-1716 bp	0.647059	74.4
dsbD_p4	AATAGGAGCAATTCGGCCAG	GGTTCAAATCCTTCCTGGGG	50.0	55.0	58.0	58.1	1376-1403 bp	0.588235	71.3
dsbD_p5	AGCGTACAAGCTCGTTTCTT	TCAAATCCTTCCTGGGGAGT	45.0	50.0	57.8	57.9	1340-1353 bp	0.117647	48.4