Hotspot

dnaB

Genome · 153286-155076 · 1791 bp

Back to Gracilaria

Pi 0.158
MarkerSeek score 80.20
Primer available yes
Label dnaB

Feature Metadata

label_name
dnaB
feature_type
gene
region
Genome
length_bp
1791
variable_sites
877
pi
0.158
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
dnaB_p1 TCGACTCCAATATTTTGTACGGT TCAGTCCACTGCTCTACCAA 39.1 50.0 57.9 58.0 2659 bp 0.058824 17.7
dnaB_p2 TCGACTCCAATATTTTGTACGGT TCCACTGCTCTACCAACTGA 39.1 50.0 57.9 58.0 2655 bp 0.058824 17.7
dnaB_p3 TCGACTCCAATATTTTGTACGGT TTCAGTCCACTGCTCTACCA 39.1 50.0 57.9 58.0 2660 bp 0.058824 17.7
dnaB_p4 TCGACTCCAATATTTTGTACGGT AGTCCACTGCTCTACCAACT 39.1 50.0 57.9 58.0 2657 bp 0.058824 17.6
dnaB_p5 TCGACTCCAATATTTTGTACGGT TTTGAACTGGTGACACGAGG 39.1 50.0 57.9 58.1 2683 bp 0.058824 17.3