Hotspot

acpP

Genome · 116018-116281 · 264 bp

Back to Gracilaria

Pi 0.281

MarkerSeek score 77.60

Primer available yes

Label acpP

Feature Metadata

label_name: acpP
feature_type: gene
region: Genome
length_bp: 264
variable_sites: 184
pi: 0.281
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
acpP_p1	GGAGAGGTGGTAGAGTTGGT	TAAGGTCCCGTAGTTTTGGC	55.0	50.0	58.1	57.5	549 bp	0.058824	38.1
acpP_p2	CACAGGTGGAGCTGCAATAA	TAAGGTCCCGTAGTTTTGGC	50.0	50.0	58.2	57.5	973 bp	0.058824	37.1
acpP_p3	GGGATGGCTTAGATCTGCAG	TAAGGTCCCGTAGTTTTGGC	55.0	50.0	57.8	57.5	1054 bp	0.058824	37.1
acpP_p4	AGTACAGGGGGATGGCTTAG	TAAGGTCCCGTAGTTTTGGC	55.0	50.0	58.2	57.5	1062 bp	0.058824	37.0
acpP_p5	GTACAGGGGGATGGCTTAGA	TAAGGTCCCGTAGTTTTGGC	55.0	50.0	58.2	57.5	1061 bp	0.058824	37.0