Hotspot

syfB-rps1

Genome · 160535-161064 · 530 bp

Back to Gracilaria

Pi 0.101

MarkerSeek score 82.30

Primer available yes

Label syfB-rps1

Feature Metadata

label_name: syfB-rps1
feature_type: igs
region: Genome
length_bp: 530
variable_sites: 171
pi: 0.101
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
syfB-rps1_p1	TTTTGCGAAGATCGTGGTCA	CCTGCTATGCTTACTCCGAC	45.0	55.0	58.1	57.9	1276-1290 bp	0.117647	47.2
syfB-rps1_p2	GCGAAGATCGTGGTCACATA	CCTGCTATGCTTACTCCGAC	50.0	55.0	57.8	57.9	1272-1286 bp	0.117647	46.6
syfB-rps1_p3	TTTTGCGAAGATCGTGGTCA	ACCTGCTATGCTTACTCCGA	45.0	50.0	58.1	58.2	1277-1291 bp	0.117647	46.5
syfB-rps1_p4	GCGAAGATCGTGGTCACATA	ACCTGCTATGCTTACTCCGA	50.0	50.0	57.8	58.2	1273-1287 bp	0.117647	45.9
syfB-rps1_p5	TGTGACTTCGTTTGAGTGCT	CCTGCTATGCTTACTCCGAC	45.0	55.0	57.7	57.9	1343 bp	0.058824	38.7