Hotspot

orf196-trnM(cat)

Genome · 70374-70985 · 612 bp

Back to Gracilaria

Pi 0.109

MarkerSeek score 85.10

Primer available yes

Label orf196-trnM(cat)

Feature Metadata

label_name: orf196-trnM(cat)
feature_type: igs
region: Genome
length_bp: 612
variable_sites: 233
pi: 0.109
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf196-trnM(cat)_p1	TGCTCTATTTGATCCCACGG	TCCTTCAGCTGTTGCAACTT	50.0	45.0	57.4	57.9	800 bp	0.058824	36.9
orf196-trnM(cat)_p2	GCTCTATTTGATCCCACGGT	TCCTTCAGCTGTTGCAACTT	50.0	45.0	57.4	57.9	799 bp	0.058824	36.9
orf196-trnM(cat)_p3	TGCTCTATTTGATCCCACGG	TTTCATTCTCCCCATGGCTG	50.0	50.0	57.4	57.9	961 bp	0.058824	36.2
orf196-trnM(cat)_p4	GCTCTATTTGATCCCACGGT	TTTCATTCTCCCCATGGCTG	50.0	50.0	57.4	57.9	960 bp	0.058824	36.2
orf196-trnM(cat)_p5	GCTAGAGGCTGTACATCGTT	TCCTTCAGCTGTTGCAACTT	50.0	45.0	57.1	57.9	1376 bp	0.058824	34.9